Question

Which enzymes are needed to produce recombinant plasmids to be used in gene transfer?

A. DNA polymerase and DNA ligase
B. DNA polymerase and restriction enzyme (endonuclease)
C. Transcriptase and RNA polymerase
D. Restriction enzyme (endonuclease) and DNA ligase

Answer 1

D. Restriction enzyme (endonuclease) and DNA ligase

Step-by-step explanation:

Hope this helps

Answer 2

The answer is D. For producing recombinant, first you need to cut the insert part and vector part to get the same end by using restriction enzyme. Then using DNA ligase to bind these two parts to get a new plasmids.