Ok, so I'll be quantizing time here to get you the most accurate result I can give you.
What you have to know is that:

Also:
t=time snapshot (in minutes)
x=distance first ant has travelled
y=distance second ant has travelled (perpendicular to the first ant)
D=distance between both ants
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Now, when:
t=0, x=0, y=0, D=0
t=1, x=4, y=0, D=4
t=2, x=8, y=0, D=8
t=3, x=12, y=5, D=13
Now we quantize time and find the tangent line (slope) between two points on the distance vs time graph at t=3 and t=3.000001, knowing that distance is represented by feet and time is represented in minutes.
When t=3.000001, x=12.000004, y=5.000005 D=13.00000562...
Now we find the slope on the distance vs time graph between the points (3,13) and (3.000001, 13.00000562).

So, at the point (3,13) on the distance vs time graph, the two ants are moving away from each other at approximately
5.62 feet per minute.
Notations you may require to understand problem in the attachment below.