Question

Sometimes, samples can be placed directly under a microscope. At other times, it is necessary to add water to the slide and make a wet mount. The steps for preparing a wet mount are shown below. In what order should these steps be followed? If necessary, remove excess water by holding a paper towel next to the cover slip, or add more water by placing another drop next to the cover slip. Use an eyedropper to place a drop of water on the sample. Place a sample on a slide. Place one end of a cover slip on the slide and slowly lower the other end.

Answer 1

Microscopic slide is a transparent object made of plastic or glass which is used to support the sample.

Slide preparation of a microscopic sample is a crucial step which depends on the nature of the specimen.

In the given question, the steps have been described to prepare a wet mount of the sample which is performed as followed:

1. Place a drop of liquid using in the center of the slide using the eyedropper. Place a sample on a slide.

2. Place the sample on the liquid if sample not present in liquid.

3. Cover the sample with coverslip by holding coverslip from one end and slowly lower the cover slip.

4. Remove extra liquid if required by holding a piece of paper to the coverslip.

5. Add more liquid by adding drop next to the coverslip.

Answer 2

Place a sample on a slide. Use an eyedropper to place a drop of water on the sample. Place one end of a cover slip on the slide and slowly lower the other end. If necessary, remove excess water by holding a paper towel next to the cover slip, or add more water by placing another drop next to the cover slip.