Answer:
The closing of the valves between the atria and ventricles.
Step-by-step explanation:
Heart sounds are defined as noises made by the beating heart and blood flow through heart. The S1 or first heart sound is generated by turbulence due to closing of two components, mitral valve and tricupsid valve.
The mitral valve and tricupsid valve are generally known as atrioventricular valves as they present between the atria and ventricles.
S1 sound is has low frequency, which is described as 'lub' and generates at the beginning of systole
Thus, the correct answer is third option.