Final answer:
The pre-Columbian event is when the Native American population reached 50 million people, before the arrival of Columbus and the subsequent European colonization and spread of diseases.
Step-by-step explanation:
The pre-Columbian period refers to the time before the arrival of Columbus and European influence in the Americas. Looking at the options provided, the event that is pre-Columbian would be when the Native American population reached 50 million people. This occurred before the devastating impacts of European invasion, such as the introduction of new diseases to which Native Americans had no immunity, which led to a massive decline in their populations. Horses were introduced by the Spanish after Columbus, so any options referencing them or interactions with the Spanish (such as shared tools) are post-Columbian events.