Question

Spectroscopy measurements may be made in either percent transmittance (% t) or absorbance (

a. based on the mathematical relationship between absorbance and transmittance, a= -log t, explain why calibration curves of absorbance versus concentration may deviate from a straight line when a<0.1 and when a>1.

Answer 1

The calibration curves versus the concentration may deviate from the straight line when a< 0.1 and when a >1 because at these times the relationship between the absorbance and the transmittance will be an inverse relationship i.e a = log t and the substance will react differently to light at these points

Explanation:

The measurement used in the spectrum of light through substances depends on the transmittance, absorbance/ concentration of the substances when a light ray is passed through it.

This kind of measurement are practically carried out in a lab and are called spectroscopy measurements. they are done in ideal conditions hence they are carried out in a lab. the value of absorbance largely determines if the graph of absorbance versus concentration will be a straight line graph or not.