Answer: He should have used a large sample size.
Johannes wanted his experiment to be improved, this can be done only when the experiment will be conducted with a large sample size.
Any experiment should be conducted based on the fact that the sample size should be large.
Here, in this experiment Johannes used only a single tree for five years. He should have used large sample size for more accurate result.
The effect of soil, water or both can be studied when there is a control and a experimental sample.