Question

Explain why a plasmid and a segment of DNA to be inserted into a plasmid must be cut with the same restriction enzyme. Use the term sticky ends in your response.

Answer 1

They have to be cut with the same restriction enzyme because the DNA needs to fit into the plasmids sticky ends so that they can be glued together for a plasmid.

Answer 2

The sticky ends have to match so ligase can do its job.