Question

You put a human gene into a yeast cell to be expressed. Because yeast are eukaryotes, you expect everything to be fine. But then, some of your yeast begin making protein that is way too long! You sequence their DNA, and you find a mutation.Which of the following mutations could have caused the protein to be too long? Choose all that apply.Choice 1 of 4:A mutation that caused RNA polymerase to keep going after it normally stopsChoice 2 of 4:A mutation that changed a STOP codon to a non-STOP codonChoice 3 of 4:A mutation that prevented splicing from occurring at a particular location

Answer 1

The likely causes of the abnormal protein could be, both choice 1 and choice 2. Both are pretty similar in their consequence, since they describe a mutation which either keeps RNA polymerase to keep going after it usually stops or the stop codon, is not a stop site anymore. This causes are well documented and some of them are causes of illness. The third option, although feasible, it would find many obstacles, since introns would be hard to traduce. So, to sump up, choice 1 and choice 2 are the correct options.