Question

This is known as a beer's law plot, the expression for which is a = e*c. a is the absorbance, c is the concentration, and e* is the effective molar absorptivity which is the slope of a beer's law plot. the slope must be accurately determined because it will enable you to solve for the concentration of your bromothymol blue solution of unknown concentration.

Answer 1

Beer's Law is a principle used in chemistry to determine the concentration of a substance in a solution based on its absorbance of light. The expression for Beer's Law is a = e * c, where a is the absorbance, c is the concentration, and e is the effective molar absorptivity, which is the slope of a Beer's Law plot.

Step-by-step explanation:

Beer's Law is a principle used in chemistry to determine the concentration of a substance in a solution based on its absorbance of light. The expression for Beer's Law is a = e * c, where a is the absorbance, c is the concentration, and e is the effective molar absorptivity, which is the slope of a Beer's Law plot. By accurately determining the slope, you can solve for the concentration of a solution of unknown concentration. This is commonly used in spectrophotometry and can be applied to various compounds, such as DNA, RNA, and proteins.

Answer 2

Enter the value of your slope to 3 significant figures and in units of L/µmol.

Slope (L/µmol) =

Your Answer:0.0295