Question

The final step of the cloning procedure is to screen the plasmid library for clones that carry the vgp gene, shown in red below. to accomplish the screening, researchers synthesize a single-stranded dna probe using vgp mrna as a template. what will be the sequence of nucleotides in the probe? drag the labels to their appropriate locations in the probe. labels can be used once, more than once, or not at all

Answer 1

Screening the plasmid library is TCACACTG...GAATTCAT-3'

The ultimate measure of the cloning system is to select the plasmid library for clones that take the VGP gene and displays in red. To perform the screening, researchers integrate a single-stranded DNA exploration applying VGP on the MRNA as a sample.

Answer 2

To screen a library of bacterial states for clones that convey a particular quality, a moderately short, single-stranded nucleic corrosive test is hybridized to the DNA of that quality. Regularly, the test is a DNA strand orchestrated by turn around transcriptase from the mRNA encoded by the quality.
Bases in the test hydrogen-cling to correlative bases in the quality. On the off chance that the test has been marked with a radioactive isotope or a fluorescent tag, analysts can distinguish the bacterial clones that contain the DNA to which the test has bound. Those clones can be developed in huge amounts, enabling many duplicates of the quality to be separated for use in examine or different applications.