Question

It contains a bamh1 site (g^gatcc) and a bglii site (a^gatct). in order for the synthetic gene to be inserted between these two sites, it should be constructed to have which restriction sites at its beginning and at its end? select all that apply.

Answer 1

The answers are:

BamH1 (G^GATCC), followed by BamH1 (G^GATCC)

BamH1 (G^GATCC), followed by BglII (A^GATCT)

BglII (A^GATCT), followed by BglII (A^GATCT)

Since the similar sticky ends are made by both BamH1 and BglII sites can be positioned at both ends of the synthetic gene. DNA fragments made by the same enzyme can permanently be ligated.