In the b-galactosidase lab, why was o-nitrophenol-b-d-galactosidase (onpg) used?

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asked Jan 11, 2018 223k views

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In the b-galactosidase lab, why was o-nitrophenol-b-d-galactosidase (onpg) used?

Biology
high-school

Ahmed Niri asked

by Ahmed Niri

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When we use ONPG, we are able to evaluate b-galactosidase activity.

Uzoma answered Jan 15, 2018

by Uzoma

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Beta-galactosidase follows Michaelis-Menten kinetics. A 5 micromolar solution of beta-galactosidase catalyzes the formation of 0.8 mmoles ortho-nitrophenol s-1 when saturated with the substrate ortho-nitrophenyl-beta-galactoside.

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