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If dye x has a molar absorptivity, ε = 4.50 x 104 cm-1 m-1, at a certain wavelength, what concentration of the dye, in a 1.00 cm cuvette cell will produce an absorbance of 0.417?
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Oct 11, 2018
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If dye x has a molar absorptivity, ε = 4.50 x 104 cm-1 m-1, at a certain wavelength, what concentration of the dye, in a 1.00 cm cuvette cell will produce an absorbance of 0.417?
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Petershine
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For this problem, the working equation should be used from the Beer's Law:
A = ∈lc,
where
A is the absorbance
∈ is the molar absorptivity
l is the length of path of the cuvette diameter
C is the concentration of the sample placed inside the cuvette
Substituting the values:
0.417 = (4.50×10⁴ cm⁻¹ M⁻¹
)(1 cm)(C)
Solving for C:
C = 9.27
×10⁻⁶ M
Zach Hirsch
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Oct 17, 2018
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Zach Hirsch
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