Final answer:
Chromosomes contain a single DNA strand after the sister chromatids separate during anaphase of mitosis, transitioning from a duplicated state with two strands.
Step-by-step explanation:
Chromosomes with a Single Strand of DNA
DNA replication occurs in the S phase of interphase, setting the stage for mitosis. However, chromosomes contain a single strand of DNA only after the sister chromatids separate, which occurs during the anaphase of mitosis. After this separation, each individual chromatid is now referred to as a chromosome, containing a single strand of DNA. The four phases of mitosis are prophase, metaphase, anaphase, and telophase. Prior to anaphase, each chromosome is composed of two sister chromatids held together at the centromere.