The second option, "Use the subject line wisely to let your reader know the main purpose of the e-mail," is most appropriate.
You can find this answer by eliminating the options -- the first option doesn't make sense because if you include all the details in the subject line, it will be too long. The third option, one or two words, would not help the reader know what's in the email. And the last option, putting nothing in the subject line, would also not help the reader know what's in the email.