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The two upper chambers of the heart are separated from each other by the

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 lungs or ribs that separate the two upper chambers from each other
User Priyank Gupta
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The upper chambers, called atria, are separated by the interatrial septum. The lower chambers, called ventricles, are separated by the inter ventricular septum. More generally these are called septa, or partitions. Ribs are outside of the heart, as are the lungs.
User AlexeyDaryin
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