If an expression is the difference of two squares, it will follow this format:

where a and b are integers or variables.
1)

Since the expression follows the format, it's a DoTS.
2) Since the binomial has an addition sign, it isn't a DoTS.
3)

The second term isn't an integer, so the expression isn't a DoTS.
4)

Since the expression follows the format, it's a DoTS.
Therefore, the first and fourth expressions are differences of two squares.