Question

Unstained cells have very little contrast. What single adjustment could you make to your microscope that would make cells easier to see at the same time while reducing the evaporation of your sample?

Answer 1

Answer and Explanation:

Microscope objects have thermal profiles. These profiles differ as a purpose of their physical parameters. Most objects are sold and designed for the resolution of fixed cell microscopy.

There is little indication that the temperature cycle affects the strain characteristics. A large box can be constructed around the microscope and heated with hot air.

The heating system's cycling causes the specimen to drift out of focus and can also change the coverslip position.

The microscope can be equilibrated to a single temperature to eliminate the movement resulting from the thermal expansion of the microscope's components.

Air currents connecting the specimen chamber should also be reduced—tight control of temperature for the entire laboratory and microscope. Any change in the temperature can lead to unwanted movement in the microscope. Heating ducts often produce localized temperature changes.