As a protocol, the sample must first undergo primary biochemical tests, one of which is gram staining. And although this stain can help us to identify bacteria of the Clostridium genus, such as gram + sporulated bacilli, a more specific test would be the endospore stain, since the clostridium genus produces endospores that are also easy to isolate, since they are more resistant to temperature than the vegetative form (not sporulated) of the bacterium. An example would be the Schaeffer-Fulton stain.