Answer:
The barriers could be:
-Control of plasmid replication by antisense RNA.
-Control of plasmid replication by proteins and repeated sequences.
-Incompatibility mediated by partition systems.
Step-by-step explanation:
Plasmids are genetic elements that can often be a strain on the bacteria. To prevent the elimination of the plasmid, they have a control system that has to do with the number of copies. These systems respond and control to the changes that can occur in an increase in the number of copies as well as in their decrease. The systems described above are primarily responsible for plasmid incompatibility.