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Serine has pka1 = 2.21 and pka2 = 9.15. use the henderson-hasselbalch equation to calculate the ratio neutral form/protonated form at ph = 3.05.
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Serine has pka1 = 2.21 and pka2 = 9.15. use the henderson-hasselbalch equation to calculate the ratio neutral form/protonated form at ph = 3.05.

User Wayne See
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Calculate the ratio by using Henderson-Hasselbalch equation:

pH = pKa + log [neutral form] / Protonated form

3.05 = 2.21 + log [neutral form] / [Protonated form]

3.05 - 2.21 = log [neutral form] / [Protonated form]

0.84 = log [neutral form] / [Protonated form]

[neutral form] / [protonated form] = anti log 0.84 = 6.91
User Bassfader
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