Final answer:
Spermatogonia are located closest to the outer edge of the seminiferous tubules, where they initiate the process of spermatogenesis before cells move inward toward the lumen.
Step-by-step explanation:
Spermatogonia in the seminiferous tubules are located closest to the outer edge of the tubule. This location facilitates the process of spermatogenesis, which begins with these diploid stem cells known as spermatogonia. The spermatogonia undergo mitosis, with one cell continuing as a stem cell and the other becoming a primary spermatocyte that will undergo meiosis to form sperm. During the progression of spermatogenesis, cells move inward toward the lumen as they develop. Sertoli cells, which act as nursemaid cells, and Leydig cells, which produce testosterone, assist in the process and are found within the walls of the seminiferous tubules.