Final answer:
The major assumption when drawing evolutionary relationships between organisms is that more closely related organisms will look more alike, although this is not always true due to environmental factors and adaptations.
Step-by-step explanation:
A major assumption when drawing evolutionary relationships between organisms is that more closely related organisms will look more alike. However, this assumption is not always true. If two closely related lineages evolved under significantly different surroundings or after the evolution of a major new adaptation, they may look quite different from each other, even more so than other groups that are not as closely related. For example, a phylogenetic tree may show that lizards and frogs appear more similar than lizards and rabbits, even though lizards and rabbits are more closely related.