You have the main rule: no vowel is isolated between two consonants.
The word FIXTURE consists of 4 consonants and 3 vowels.
There are such possible cases:
1. Formed word begins with three vowels and ends with 4 consonants.
The number of such words is

2. Formed word begins with two vowels and ends with one vowel (between them stand all consonants).
The number of such words is

3. Formed word begins with one vowel and ends with two vowels (between them stand all consonants).
The number of such words is

4. Formed word begins with with 4 consonants and ends with 3 vowels.
The number of such words is

5. In total 144+144+144+144=576 different words.