Question

Suppose jennifer has a pcr product that contains three dna fragments of 300, 700, and 1000 base pairs in length. she separates the three fragments using gel electrophoresis. which fragment will migrate the farthest from the wells at the top of the gel?

Answer 1

The answer is the fragment with 300 base pairs length. Gel electrophoresis separates DNA fragments (and other biomolecules chains) by their lengths. On theone hand, the small fragments are able to travel fast through the pores in the gel. On the other hand, the larger the fragments, the slower they travel along the gel due to the obstacle of passing through the small pores.