Question

Explain how electrophoresis can be used as a part of genetic testing.

Answer 1

During genetic testing, a segement of amplified DNA obtained from gene is digested using a restriction enzyme. Electrophoresis allows one to analyze the patterns by separating DNA by size. The process allows separation of different sizes of DNA fragments and provides genetic information in a wide range of data fields. A good example is genetic testing; Human DNA can be analyzed to provide evidence in criminal cases, to diagnose genetic diseases, and to solve partenity cases.

Answer 2

Electrophoresis separates polypeptide chains and DNA chains by their fragment sizes after they have been cut by restriction enzymes are specific sites. Genetic testing involves checking genetic disorders by evaluating DNA sequences or protein amino acid sequences. By using restriction enzymes to cut DNA or polypeptides at specific sites, then run them on an electrophoresis, bands will form that will help indicate whether one has an abnormal sequence at the target site. If so, further analysis is used to determine the sequence by use of next-generation sequencing machines