Question

Briefly explain the steps in the process of removing the human gene and inserting it into plasmid DNA

Answer 1

Plasmids are extracted from the bacteria

The plasmids are spliced at specific regions using restriction enzymes.

The cut plasmids are incubated with the novel gene and DNA ligase

Plasmids that will have incorporated the new gene are transformed back into the bacteria

Step-by-step explanation:

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Answer 2

)Insert gene of interest into plasmid
2)recombinant DNA is placed back into bacteria
3)bacteria divides
4)gene is now expressed in bacteria and product can be harvested