Question

Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. the use of mrna and reverse transcriptase is part of a strategy to solve the problem of _____.

Answer 1

Out of the flowing given choices;

A) post-transcriptional processing.

B) electroporation.

C) post-translational processing.

D) nucleic acid hybridization.

E) restriction fragment ligation.

The answer is A. Bacteria do not have a complex post-transcriptional processing tool such as the one in eukaryotes cells. Therefore, they are not able to process pre-mRNA with introns, example splicing. Therefore, a fully processed mRNA is cloned into the bacterial cell so it is reverse-transcribed into cDNA by reverse transcriptase.