Question

Studies of the endomembrane system often involve the use of a protein that can emit a green fluorescence (glow). a researcher wants to make a video of cell behavior, so she initially tags the outer nuclear envelope of a cell with the fluorescent tag and records for several hours. later, she sees that the tag is part of a secretory vesicle. the ability to stain protein molecules with a fluorescent dye would most clearly allow researchers to go beyond what they could previously detect with a microscope by allowing them to

Answer 1

The right answer is A) detect ribosome activity.

The endomembrane system, present only in eukaryotic cells, is the whole cytoplasmic cavities limited by membranes inter-communicating with each other via vesicles or canaliculi. The different compartments of this system are endoplasmic reticulum, the Golgi apparatus, phagosomes and endosomes, lysosomes and the plant vacuole.

The endoplasmic reticulum is a set of membranes delimiting cavities in the form of cisterns or tubules. It could be:

* Devoid of ribosomes, such as smooth endoplasmic reticulum

* Or carrier of ribosomes, it is the granular endoplasmic reticulum that is related to the nuclear envelope (where the signal was initially present, then due to its dynamic structure of the system, it displaced and transformed into secretory vesicles).

Answer 2

The answer is A: detect ribosome activity. This is because ribosomes are the main organelles that manufacture proteins in a cell. Tagging of proteins, or other biomolecules, with fluorescent dyes allows scientists to track the protein and analyze it's movements in a biological pathway or activity/interaction with other biomolecules. The fluorescent dye should not influence or affect the behaviour of the protein. It's use in microscopy is applied in Fluorescent Lifetime Imaging Microscopy (FLIM)