phosphoenolpyruvate is produced as an intermediate of glycolysis; phosphoenolpyruvate donates its high-energy phosphate group to enzyme I (EI);
EI donates the phosphate group to a histidine-rich protein (HPr);
HPr donates the phosphate group to enzyme IIA (EIIA);
EIIA passes its phosphate group to Enzyme IIB (EIIB);
as it enters the cell, glucose is phosphorylated by EIIB;
glucose is transported across the membrane by enzyme IIC (EIIC);
and the end product glucose-6-phosphate is now chemically different from the parent solute.