False.
Glycolysis is the 1st metabolic step for both aerobic and anaerobic metabolism. The process of glycolysis takes 1 glucose molecule and 2 ATP molecules, then produces 2 pyruvate molecules and 4 ATP molecules for a net gain of 2 ATP molecules. The ATP is used by the organism for immediate energy and the pyruvate is further metabolized using either an aerobic or anaerobic metabolism. If anaerobic, the pryruvate is fermented into either ethanol and carbon dioxide, or into lactic acid. The aerobic pathway is a long chain of reactions that eventually produces an additional 28 or 30 ATP molecules for the organism's energy needs.
So as you can see, both aerobic (oxygen using) and anaerobic organisms perform glycolysis.