Question

Which of the following procedures would produce RFLPs?

A) incubating a mixture of single-stranded DNA from two closely related species
B) incubating DNA nucleotides with DNA polymerase
C) incubating DNA with restriction enzymes
D) incubating RNA with DNA nucleotides and reverse transcriptase
E) incubating DNA fragments with ʺsticky endsʺ with ligase

Answer 1

Restriction fragment length polymorphism (RFLP) could be produced by incubating DNA nucleotides with restriction enzymes.