Answer is: No, because the substance could be an element.
Pure substance is made of only one type of atom (element) or only one type of molecule, it has definite and constant composition with distinct chemical properties.
Pure substances can be separated chemically, not physically, that is difference between pure substances and mixtures.
Elements (for example copper, iron, sulfur) and compounds (water, sodium chloride) have definite and constant composition with distinct chemical properties.