Question

The spectrophotometer really measures the percent of light that is transmitted through the solution. The instrument then converts %T (transmittance) into absorbance by using the equation you determined in the prelab section. If the absorbance of a sample is 0.85, what is the percent light transmitted through the colored sample at this collected wavelength?

Answer 1

Hey there!:

absorbance = log 100 - log Transmitance

absorbace = 0.85

log 100 = 2

- log transmitance = absorbace / log 100

0.85 / 2= 0.425

transmitance = 10 ^ ( - 0.425 )

transmitance = 0.376