Final answer:
In the absence of oxygen, the net gain of ATP from each glucose molecule entering glycolysis is two ATP, due to the production of four ATPs and the consumption of two during the process.
Step-by-step explanation:
The net gain of ATP for each glucose molecule that enters glycolysis, in the absence of oxygen, is approximately two ATP. During glycolysis, a single glucose molecule is broken down into two pyruvate molecules, and a total of four ATPs are produced. However, because two ATPs are consumed during the early stages of glycolysis, the net production is two ATPs. These ATPs are the result of substrate-level phosphorylation.