The correct answer to this open question is the following.
One specific way in which native peoples defended their political sovereignty after European encroachment on their lands was war. Yes, the Native American Indian tribes always resisted the white English colonist's invasions to their lands, and although sometimes they tried to be amicable and offered some help to the colonists, as was the case of the beginning of the Jamestown, Virginia colony and the Powathans tribe, the constant was aggression and war.
So we could say that the Native American Indians tried diplomacy, negotiation, sometimes alliances, and definitely, their last resource was war.