Question

If you have access to stock solutions of 1.00 M H3PO4, 1.00 M of HCl, and 1.00 M NaOH solution, (and distilled water of course), what volumes of each would you mix before diluting to a final volume of 2.00 L to prepare 2.00 L of pH 7.40 buffer with a final total concentration of 50 mM of phosphorous contains species (e.g. so that [H3PO4] [H2PO4 - ] [HPO4 2- ] [PO4 3- ]

Answer 1

0.10L of 1.00M of H₃PO₄ and 0.1613L of 1.00M NaOH

Step-by-step explanation:

The pKa's of phosphoric acid are:

H₃PO₄/H₂PO₄⁻ = 2.1

H₂PO₄⁻/HPO₄²⁻ = 7.2

HPO₄²⁻/PO₄³⁻ = 12.0

To make a buffer with pH 9.40 we need to convert all H₃PO₄ to H₂PO₄⁻ and an amount of H₂PO₄⁻ to HPO₄²⁻

To have a 50mM solution of phosphoures we need:

2L * (0.050mol / L) = 0.10 moles of H₃PO₄

0.10 mol * (1L / mol) = 0.10L of 1.00M of H3PO4

To convert the H₃PO₄ to H₂PO₄⁻ and to HPO₄²⁻ must be added NaOH, thus:

H₃PO₄ + NaOH → H₂PO₄⁻ + H₂O + Na⁺

H₂PO₄⁻ + NaOH → HPO₄²⁻ + H₂O + Na⁺

Using H-H equation we can find the amount of NaOH added:

pH = pKa + log [A⁻] / [HA] (1)

Where [A-] is conjugate base, HPO₄²⁻ and [HA] is weak acid, H₂PO₄⁻

pH = 7.40

pKa = 7.20

[A-] + [HA] = 0.10moles (2)

Replacing (2) in (1):

7.40 = 7.20 + log 0.10mol - [HA] / [HA]

0.2 = log 0.10mol - [HA] / [HA]

1.5849 = 0.10mol - [HA] / [HA]

1.5849 [HA] = 0.10mol - [HA]

2.5849[HA] = 0.10mol

[HA] = 0.0387 moles = H₂PO₄⁻ moles

That means moles of HPO₄²⁻ are 0.10mol - 0.0387moles = 0.0613 moles

The moles of NaOH needed to convert all H₃PO₄ in H₂PO₄⁻ are 0.10 moles

And moles needed to obtain 0.0613 moles of HPO₄²⁻ are 0.0613 moles

Total moles of NaOH are 0.1613moles * (1L / 1mol) = 0.1613L of 1.00M NaOH

Then, you need to dilute both solutions to 2.00L with distilled water.