Question

You need to make 200ml of a 2% agarose gel in a buffer solution (1x tae) how many grams of agarose do you need?

Answer 1

The number of grams of agarose that you need to make 200 ml of 2% agarose gel is 4 grams


calculation
number of grams is calculated using M/v x100 =% m/v formula
M (mass) =?
V(volume) = 200 ml
%(M/V) = 2%

convert the 2 % M/V into fraction = 2/100

then substitute the values into the equation

= M/200 ml = 2/100

multiply both side by 200

M is therefore = 2/100 x200

M=4 grams