A Gram- negative bacteria has a thin cell wall of Peptidoglycan which is sandwiched between the cell membrane and outer membrane. Where as, the Gram positive bacteria has a thick layer of Peptidoglycan outside their cell membrane.
Hence Gram- negative bacteria is different from a Gram- negative bacteria in because it has an extra layer that is outside the Peptidoglycan layer.
Hence the right option is D
This feature of the Gram-negative bacteria does not let crystal violet solution to soak into its Peptidoglycan layer because it is covered by the extra layer where as an Gram- positive bacteria will easy soak into crystal violet solution due to the exposed Peptidoglycan layer.