RFLP = Restriction fragment length polymorphismWe can say that an individual has a recombination when we see that in RFLP there are two bands. One in normal migration, and one in the pathologic migration.In generation III we can see it in individual 1, 3, 5, 7, 8, and 10. Any modification of the DNA sequences (mutation, addition, deletion) frequently rearranges the restriction sites. During the action of restriction enzymes, the size of the restriction fragments is then modified, and the fragments are then separated differently according to their size by electrophoresis: a polymorphism is observed.