The correct answer is option 1.
After the initial settlement, the Jamestown colonists and the neighboring Powhatan tribes were often in conflict over land. During the "starving time," colonists raided the Native American tribes and took their food supplies. As a result, permanent attacks were held on both sides for the next few years. Finally, in 1614, Powhatan accepted to make peace with the English. When Powhatan's daughter, Pocahontas, was married to John Rolfe and taken to England, she died of disease three years later. As a consequence, Powhatan died in despair in 1618 and his brother decided to continue the fight against the English. When Powhatan's brother was captured and executed in 1645, the English forced the tribes to cede land and recognize English authority.