Question

Resolution in chromatography is a function of three fundamental parameters. These are

a.) peak height, peak area, and peak position

b.) retention time, retention volume, and peak area

c.) column pressure, column length, and peak height

d.) selectivity, number of plates, and capacity factor

Answer 1

Resolution in chromatography is a function of "selectivity, number of plates, and capacity factor".

Step-by-step explanation:

Resolution is a measure of the separation of 2 peaks of different retention time t in a chromatography divided by the combined widths of the elution peaks.

The resolution is denoted as a numerical value, such as 0.3, 4.0, or 1.0

. Resolution not only depend on column, but also on the flow-rate and mobile phase composition, the rate of gradient and the composition and size of peptides themselves.

`R_(s)=(1)/(4) * (\alpha-1)/(\alpha) * (k)/(k+1) * \sqrt{N_(t h)}`

Where

`R_s` is the resolution factor.

`\alpha` is selectivity factor.

K is the retention factor or the capacity factor.

`N_(th)` theoretical plate number.