Answer:
It can cause a significant negative effects on certain tissues and target cells.
Step-by-step explanation:
Generally, based on the mechanism of C5M, its presence in any experiment can harm certain tissues and target cells. Therefore, the use of a large amount (in terms of concentration above 2 µM) of C5M will enhance the negative effects of C5M on the surrounding/neighboring tissue as well as the normal division of target cells. That is the reason why a higher concentration was not used in the experimental runs.