Question

ELISA immunoassay experiment

1. The samples that youa dded to the microplate strip contain many proteins and may or may not contain the antigen. What happened to the proteins in the plastic well if the sample contained the antigen? What if it did not contain the antigen?

2. When you added primary antibody to the well, what happened if your sample contained the antigen? what if it did not contain the antigen?

Answer 1

If contain, the colorant stays, if not, it washes.

Step-by-step explanation:

The ELISA inmmunoassay experiment consists on adding to an antibody a sample with a colorant, if the sample contains an antigen, it will stick to its proper antibody and te colorant will remain. If not, when the laboratory worker washes the sample after adding the sample, the colorant and the sample will be gone because there wasn´t an antigen on the sample that fitthe antibody. In that case, the result will be negative.