Question

To determine the isoelectric point of a protein, first establish that a gel: A) contains a denaturing detergent that can distribute uniform negative charges over the protein surface. B) exhibits a stable pH gradient when ampholytes become distributed in an electric field. C) is washed with an antibody specific to the protein of interest. D) neutralizes all ionic groups on a protein by titrating them with strong bases. E) relates the unknown protein to a series of protein markers with known molecular weights.

Answer 1

The correct answer is B.

Step-by-step explanation:

To determine the isoelectric point of a protein, first establish that a gel exhibits a stable pH gradient when ampholytes are distributed in an electric field. The isoelectric point is the pH at which a polyampholyte has a net charge of zero. At this pH value, the solubility of the substance is almost zero. Thus, the gel must show a stable pH gradient.

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