Question

In order to insert a human gene into a plasmid, both must _____1. have identical DNA sequences2. originate from the same type of cell3. code for the same gene product4. be cut by the same restriction enzyme5. be the same length

Answer 1

4. be cut by the same restriction enzyme

Step-by-step explanation:

Restriction enzymes are the endonucleases and cut the DNA molecules at specific sites. These sites are unique for each restriction enzyme and are called restriction sites. Since restriction enzymes cut DNA only at their respective restriction sites, digestion of two DNA molecules with the same restriction enzyme creates DNA fragments with complementary ends.

The restriction digested DNA fragments then can be ligated with each other with the help of enzyme DNA ligase. Therefore, to insert a human gene into a plasmid, both must be digested with the same restriction enzyme to create restriction fragments with complementary ends which in turn would facilitate their ligation.