Question

When the antibiotic valinomycin is added to actively respiring mitochondria, several things happen: the yield of ATP decreases, the rate of O2 consumption increases, heat is released, and the pH gradient across the inner mitochondrial membrane increases. Explain these observations by listing the causal order of events that occur beginning with the addition of valinomycin to the respiring mitochondria and ending with the release of heat. Note: some events listed do not occur and should not be placed Valinomycin added The rate of ATP synthesis decreases The rate of electron transfer and oxygen consumption increases The pH gradient across the The valinomycin-K complex moves K ion out of the mitochondrial matrix. The electrical potential across the The rate of ATP hydrolysis increases The electrical potential across the mitochondrial membrane decreases. Valinomycin binds K" ion. The valinomycin-K' complex moves into the mitochondrial matrix. The pH t across the Heat released

Answer 1

Valinomycin is an example of UNCOUPLERS.

Uncouplers generally usually inhibit the coupling between the electron transport chain and oxidative phosphorylation of ADP... As result,ATP synthesis is stopped. Vancomycin-k complex inhibit ATPs synthesis by preventing the proton motive force for supplying energy needed for phosphorylation of ADP to ATP

◼Valinomycin added

◼Valinomycin binds K" ion.

◼ The valinomycin-K' complex moves into the mitochondrial matrix.

◼The electrical potential across the mitochondrial membrane decreases

◼The rate of ATP synthesis decreases

◼The rate of electron transfer and oxygen consumption increase.

◼The valinomycin-K complex moves K ion out of the mitochondrial matrix.

◼This result in a drop in pH, with increase in heat released.

Answer 2

Question lack some information i have added the full question in ask for detail section. As this question is about listing the process i have created steps for better understanding.

Step-by-step explanation:

INITIAL STEP 1

Valinomycin added

STEP 2

Valinomycin binds K" ion

STEP 3

The electrical potential across the mitochondrial membrane decreases

STEP 4

The rate of ATP hydrolysis increases

STEP 5

The rate of ATP synthesis decreases

STEP 6

The pH gradient across the mitochondrial membrane increases

STEP 7

The electrical potential across the mitochondrial membrane decreases

STEP 8

The valinomycin-K' complex moves into the mitochondrial matrix

STEP 9

The valinomycin-K complex moves K ion out of the mitochondrial matrix

STEP 10

The rate of electron transfer and oxygen consumption increases

FINAL STEP

Heat released