Final answer:
The true statement about DNA replication is that prokaryotes have only one origin of replication. Eukaryotic cells have multiple linear chromosomes with multiple origins of replication, and DNA replication occurs simultaneously at several points.
Step-by-step explanation:
The statement about DNA replication that is true is D. Prokaryotes only have one origin of replication to initiate replication. Prokaryotic chromosome replication begins at a single point in the circular chromosome and proceeds bi-directionally around the circle. Contrary to what's stated in option C, eukaryotes do not have a single circular chromosome; rather, they contain multiple linear chromosomes. In eukaryotic cells, each chromosome has multiple origins of replication, allowing simultaneous replication at several points along the chromosome.
It's also important to note that DNA replication in prokaryotes happens in the cytoplasm, not in a nucleus, because prokaryotes lack membrane-bound organelles such as a nucleus. Moreover, eukaryotic DNA is packaged around histone proteins, whereas in prokaryotes, supercoiling managed by DNA gyrase helps in packing the DNA.