Question

You are adjusting pH in the fed-batch bioreactor using a 1.5 Molar solution of Ca(OH)2 (Assume that in given conditions the base dissolves completely). In addition, a feed solution with an osmolarity of 1500 mOsm is used for fed-batch operation. The starting cell culture medium has an osmolarity of 320 mOsm. If the total base addition was 30 mL/L of culture and if the total feed was 300 mL/L of the culture, estimate the final osmolarity.

Answer 1

Final osmolarity = 866 mOsm

Step-by-step explanation:

Starting cell culture medium = 320 mOsm

30 mL of 1600 mM NaOH into 1 L= (30 mL/1000) x 1600 = 48 mM

Osmolarity = osmoles / 1 L = (Mole x equivalent) / L = 48 x 2 = 96 mOsm

Feed solution = (300 mL / 1000 mL) 1500 mOsm = 450 mOsm

Total osmolarity = 320 + 96 + 450 = 866 mOsm

Hope this helps!